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A comparative account of primer designing resources, feature-validations and application-based categorizations

Mprime

Tool nameMprime
URLhttp://kbrin.a-bldg.louisville.edu/Tools/MPrime/
Important features1. Designs multiple primers for genes in human, rat, mouse, drosophila, and zebra fish. 2. Allows the user to select one or more inputs provided by the user to design primers for a single or multiple targets.
CitationsRouchka EC, Khalyfa A, Cooper NG. MPrime: efficient large scale multiple primer and oligonucleotide design for customized gene microarrays. BMC Bioinformatics. 2005 Jul 13;6:175. PubMed PMID: 16014168.
Year of publication2005
Rank by usage frequency100
Comments
FunctionMicroarray probes, General PCR, Primer specificity checking
CategoryOnline/downloadable, Free
Features1. Checks for primers specificity against mouse, rat, fungus, and fly. 2. Allows the user to provide multiple inputs such as gene name, accession number, etc. or user can paste the sequence and the tool provides the complete information of the given inputs such as description of the target, locus etc. It directly links to the Entrez database. 3. Retrieves unigene entry for the each inputs.
Description

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